SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Dias,L.M.K.; Sales,J.N.S.; Viau,P.; Barros,M.B.P.; Nicolau,S.S.; Simões,L.M.S.; Alves,N.G.; Alonso,M.A.; Valentim,R.; Oliveira,C.A..

ABSTRACT The aim of this study was to evaluate hCG treatment on ovarian response and on pregnancy rate using a 9-day oestrus synchronization protocol in Santa Ines ewes. On a random oestrus cycle day, ewes received an intravaginal progesterone device (Primer-PR®, Tecnopec, Brazil). Nine days later (Day 9), 30µg of d-cloprostenol (Prolise®, Syntex, Argentina) and 250IU of eCG (Folligon®, Intervet, Brazil) were administered and the progesterone device was removed. This moment, the ewes were randomly assigned on two groups: Control Group and hCG Group. In the hCG Group, the ewes received 500IU of hCG (Vetecor®, Hertape-Calier, Spain) 24h after device removal. In the Control Group, the ewes did not receive any ovulation inductor. Control and hCG Groups ewes...

Aspectos morfológicos, morfométricos e histoquímicos dos túbulos armazenadores de espermatozoides da fêmea do peru Meleagris gallopavo

Palavras-chave: Progesterone; Follicle; Ovulation; Synchronization.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100122

Comparison of serum hormone levels of captive and free-living maned wolves Chrysocyon brachyurus

Miranda,J.L.P.S.; Oliveira,C.A.; Mahecha,G.A.B..

Fragmentos da junção uterovaginal de 16 exemplares de fêmeas adultas de peru foram examinados, com o objetivo de determinar as modificações morfométricas e histológicas dos túbulos armazenadores de espermatozoides (SST), durante os períodos de atividade e repouso reprodutivo. Os estudos foram realizados a partir de dissecações do oviduto esquerdo, utilizando-se técnicas histológicas, histoquímica e histométricas de rotina. Essas estruturas originam-se de invaginações do epitélio das pregas da região uterovaginal e apresentam-se tubulosas, simples e não ramificadas, com epitélio formado por células prismáticas estreitas, claras e escuras, com núcleo vesiculoso basal e microvilos apicais. As secreções dessas células apresentaram reações negativas para PAS e...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Peru; Fêmea; Túbulos armazenadores; Espermatozoide; Morfometria.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100011

Correlação entre contagens de células somáticas determinadas no "Somacount 300" utilizando-se os detergentes RBS 35 e Extram MA 01 alcalino

Maia,O.B.; Jácomo,A.T.A.; Bringel,B.A.; Kashivakura,C.K.; Oliveira,C.A.; Teodoro,L.O.F.; Silveira,L.; Teixeira da Costa,M.E.L.; Malta,M.C.C.; Furtado,M.M.; Torres,N.M.; Mattos,P.S.R.; Viau,.P; Lima,T.F.G.; Morato,R.G..

Serum hormone levels were compared between captive and free-living maned wolves and seasonal variations of sex hormones were studied. Blood samples were collected from 16 male and 26 female adult animals from Brazilian zoos, and from 30 male and 24 female free-living adults to determine serum progesterone and testosterone by radioimmunoassay. Serum testosterone concentrations varied (P < 0.05) across seasons for 16 captive males, being higher in autumn (2184.7 ± 355.1 pg/mL) than in summer (1080.7 ± 205.4 pg/mL), winter (1270.1 ± 276.6 pg/mL) and spring (963.9 ± 248.1 pg/mL), although they did not differ between summer, winter and spring. Testosterone concentration of 30 free-living males differed (P < 0.05) between autumn (824.1 ± 512.2 pg/mL),...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Maned wolf Chrysocyon brachyurus; Progesterone; Testosterone; Radioimmunoassay; Reproduction.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200015

Early effects of estrogen on the rat ventral prostate

Pereira,A.R.; Oliveira,C.A.; Marques,D.C.; Machado,P.F.; Sarriés,G.A..

The objective of this work was to compare the values of milk somatic cell counts (SCC) using the Somacount 300, and two detergents for sample preparation. The equipment was calibrated using the detergent RBS 35. Forty independent milk samples were analyzed. Each sample was randomly divided into two subsamples, and each subsample was analyzed after preparation with either RBS 35 or Extram MA 01 Alkaline detergents. The correlation coefficient between the two methods was high (0.998). This finding suggests the possibility of substitution of RBS 35 for an economical alternative.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Leite; Contagem de células somáticas; Detergente; Correlação.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300008

García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F..

Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...

Efeitos cardiorrespiratórios da metadona, pelas vias intramuscular e intravenosa, em cadelas submetidas à ovariossalpingo-histerectomia

Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002

Pereira,D.A.; Marques,J.A.; Borges,P.A.; Batista,P.A.C.S.; Oliveira,C.A.; Nunes,N.; Lopes,P.C.F..

A metadona é um opioide pouco utilizado na medicina veterinária, e há, ainda, questionamentos quanto ao seu uso. Desse modo, comparou-se o emprego da metadona, administrada pelas vias IM e IV, e avaliaram-se seus efeitos sobre as características cardiorrespiratórias e os tempos de extubação e recuperação em cadelas submetidas à ovariossalpingo-histerectomia. Utilizaram-se 16 cadelas, pré-medicadas com levomepromazina, 0.6mg/kg, IM. Após 20 minutos, a indução anestésica foi feita com propofol, 5mg/kg, IV, e a manutenção com isoflurano. Transcorridos 10 minutos, os animais receberam metadona, 0.3mg/kg, pelas vias IM - grupo GIM - e IV - grupo GIV. As mensurações das frequências cardíaca (FC) e respiratória (FR), pressão arterial média (PAM), temperatura...

Quantification of fecal estradiol and progesterone metabolites in Syrian hamsters (Mesocricetus auratus)

Palavras-chave: Cadela; Anestesia; Analgesia; Metadona; Ovário-histerectomia.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400005

Chelini,M.O.M.; Souza,N.L.; Rocha,A.M.; Felippe,E.C.G.; Oliveira,C.A..

Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal saving technique which allows longitudinal studies by permitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian hamsters during the estrous cycle...

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

Palavras-chave: Endocrinology; Fecal steroids; Syrian hamster; Reproduction; Radioimmunoassay; Estrogen.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100021

Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A..

We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....